Simian virus 40 large T-antigen function is required for induction of tetraploid DNA content during lytic infection.

Abstract excerpt

Infection of quiescent CV-1 cells with simian virus 40 mutant tsA30 at 37 degrees C resulted in the induction of two rounds of cellular DNA synthesis in T-antigen-positive cells, as previously described for wild-type simian virus 40. Following infection with tsA30 at 40.5 degrees C, T-antigen-positive cells were induced into S phase and reached a diploid G2 DNA content; however, a second S phase was not...

Topics

• Antigens, Polyomavirus Transforming
• Cell Line
• DNA
• Kinetics
• Mutation
• Polyploidy
• S Phase
• Simian virus 40
• Temperature