Article
SV40 small T antigen enhances progression to >G2 during lytic infection.
Experimental cell research - 25 Aug 1999
Whalen B, Laffin J, Friedrich T D, Lehman J M
Abstract excerpt
The infection of monkey kidney (CV-1) cells with simian virus 40 (SV40) stimulates the cells into successive rounds of DNA synthesis without an intervening mitosis, leading to the acquisition of a >G2 DNA content. To elucidate the role of small t antigen in cell cycle progression and in viral replication during infection, studies were performed using an SV40 mutant (dl888) that lacks the ability to produce small...
Topics
- Animals
- Antigens, Polyomavirus Transforming
- Blotting, Western
- Cell Cycle
- Cell Cycle Proteins
- Cell Line
- Cell Nucleus
- Chlorocebus aethiops
- Cyclin D1
- Cyclin-Dependent Kinase Inhibitor p27
- DNA
- G2 Phase
- Gene Expression Regulation
- Kinetics
- Microtubule-Associated Proteins
- Mimosine
- Mutation
- Phosphorylation