Article
Mutation of a cysteine in the first transmembrane segment of Na,K-ATPase alpha subunit confers ouabain resistance.
The EMBO journal - 1 May 1992
Canessa C M, Horisberger J D, Louvard D, Rossier B C
Abstract excerpt
The cardiac glycoside ouabain inhibits Na,K-ATPase by binding to the alpha subunit. In a highly ouabain resistant clone from the MDCK cell line, we have found two alleles of the alpha subunit in which the cysteine, present in the wild-type first transmembrane segment, is replaced by a tyrosine (Y...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Cell Line
- Cloning, Molecular
- Cysteine
- DNA
- Drug Resistance
- Kinetics
- Molecular Sequence Data
- Mutation
- Ouabain
- Phenylalanine
- Polymerase Chain Reaction
- Sequence Alignment
- Sodium-Potassium-Exchanging ATPase
- Tyrosine
- Xenopus laevis