Functional expression of rat alpha 1 Na,K-ATPase containing substitutions for cysteines 454, 458, 459, 513 and 551.

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Article

Functional expression of rat alpha 1 Na,K-ATPase containing substitutions for cysteines 454, 458, 459, 513 and 551.

Biochemistry and molecular biology international - 1 Dec 1993

Lane L K

Abstract excerpt

Site-specific mutagenesis was used to prepare the following substitutions of the rat alpha 1 Na,K-ATPase: Cys 513-->Ala, Cys 454+458+459-->Ala and Cys 551-->Ser. Ouabain-resistant HeLa cell cells expressing rat alpha 1 wild type and each of the mutants were selected, indicating that the Cys-subst...

Topics

Adenosine Triphosphate
Alanine
Animals
Blotting, Western
Cysteine
DNA, Complementary
Electrophoresis, Polyacrylamide Gel
HeLa Cells
Humans
Mutagenesis, Site-Directed
Mutation
Ouabain
Rats
Serine
Sodium-Potassium-Exchanging ATPase
Transfection

Source

Open in PubMed

Identifiers

PMID: 8136699

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-8136699-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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