Article
Functional expression of rat alpha 1 Na,K-ATPase containing substitutions for cysteines 454, 458, 459, 513 and 551.
Biochemistry and molecular biology international - 1 Dec 1993
Lane L K
Abstract excerpt
Site-specific mutagenesis was used to prepare the following substitutions of the rat alpha 1 Na,K-ATPase: Cys 513-->Ala, Cys 454+458+459-->Ala and Cys 551-->Ser. Ouabain-resistant HeLa cell cells expressing rat alpha 1 wild type and each of the mutants were selected, indicating that the Cys-substituted enzymes are active. Membranes isolated from HeLa cells expressing wild-type and mutant cDNAs all exhibit...
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