Labeling of a cysteine in the cardiotonic glycoside binding site by the steroid derivative HDMA.

doi:10.1016/0014-5793(95)00637-o

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Article

Labeling of a cysteine in the cardiotonic glycoside binding site by the steroid derivative HDMA.

FEBS letters - 10 Jul 1995

Antolovic R, Schoner W, Geering K, Canessa C, Rossier B C, Horisberger J D

Abstract excerpt

The digoxigenin derivative N-hydroxysuccinimidyl digoxigenin-3-O-methylcarbonyl-epsilon-aminocaproate (HDMA) has been shown to covalently label the ouabain binding site of the Na,K-ATPase epsilon subunit [Antolovic et al. (1995) Eur. J. Biochem. 227, 61-67]. In the present study we observed both,...

Topics

Affinity Labels
Animals
Cysteine
Digoxigenin
Mutation
Recombinant Proteins
Sodium-Potassium-Exchanging ATPase
Succinimides
Xenopus

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