Article
Recombinant human bone morphogenetic protein-2 induces osteoblastic differentiation in W-20-17 stromal cells.
Endocrinology - 1 Mar 1992
Thies R S, Bauduy M, Ashton B A, Kurtzberg L, Wozney J M, Rosen V
Abstract excerpt
To better understand the in vivo bone-inductive properties of recombinant human (rh) BMP-2, we examined the ability of the protein to alter the phenotype of a bone marrow stromal cell line. W-20-17. rhBMP-2 increased alkaline phosphatase activity in W-20-17 cells in a dose-responsive manner in th...
Topics
- Alkaline Phosphatase
- Animals
- Blotting, Northern
- Bone Morphogenetic Proteins
- Cell Differentiation
- Cells, Cultured
- Cyclic AMP
- Cycloheximide
- DNA
- Dose-Response Relationship, Drug
- Enzyme Activation
- Humans
- Mice
- Osteoblasts
- Osteocalcin
- Parathyroid Hormone
- Phenotype
- Proteins