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Article

An optimized method for the chemiluminescent detection of alkaline phosphatase levels during osteodifferentiation by bone morphogenetic protein 2.

Journal of cellular biochemistry - 1 Jan 2001

Blum J S, Li R H, Mikos A G, Barry M A

Abstract excerpt

Differentiation of osteoprogenitor cells into osteoblasts is a pivotal step during the normal development and repair of bone. Upregulation of endogenous cellular alkaline phosphatase activity (AP) is a commonly used intracellular marker for the assessment of osteoprogenitor cell differentiation into the osteoblastic phenotype. Current methods for assaying AP involve colorimetric detection of the enzyme's activity...

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Identifiers

PMID: 11169737
DOI: 10.1002/1097-4644(20010315)80:4<532::aid-jcb1007>3.0.co;2-b

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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