Interaction of Tn5 transposase with the transposon termini.

doi:10.1006/jmbi.1994.1008

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Article

Interaction of Tn5 transposase with the transposon termini.

Journal of molecular biology - 14 Jan 1994

Wiegand T W, Reznikoff W S

Abstract excerpt

Transposition of Tn5 requires the binding of the transposase protein to the transposon outside end (OE) DNA sequences. Transposase mutants that increase the transposition frequency result in the formation of two distinct transposase/OE DNA complexes, observed by gel retardation analysis. The slow...

Topics

Bacterial Proteins
DNA Transposable Elements
DNA, Bacterial
DNA-Binding Proteins
Models, Genetic
Mutagenesis, Site-Directed
Nucleotidyltransferases
Phenotype
Transposases

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