Article
In vitro association of phosphatidylinositol 3-kinase activity with the activated insulin receptor tyrosine kinase.
The Journal of biological chemistry - 5 Jan 1992
Yonezawa K, Yokono K, Shii K, Ogawa W, Ando A, Hara K, Baba S, Kaburagi Y, Yamamoto-Honda R, Momomura K
Abstract excerpt
We previously have shown that insulin treatment of cells greatly increases the activity of phosphatidylinositol (PI) 3-kinase in immunoprecipitates made with an antibody to phosphotyrosine. However, the association of PI 3-kinase activity with the activated insulin receptor is not significant under these conditions. In the present study, we have attempted to reconstitute the association of PI 3-kinase activity...
Topics
- Animals
- Autoradiography
- Cattle
- Chromatography, Thin Layer
- Cricetinae
- Detergents
- Humans
- Liver
- Mutation
- Phosphatidylinositol 3-Kinases
- Phosphorylation