ras transformation of simian virus 40-immortalized rat hepatocytes: an in vitro model of hepatocarcinogenesis.

Article

ras transformation of simian virus 40-immortalized rat hepatocytes: an in vitro model of hepatocarcinogenesis.

Cancer research - 1 Jan 1992

Fang X J, Flowers M, Keating A, Cameron R, Sherman M

Abstract excerpt

Primary rat hepatocytes were transfected with simian virus 40 DNA and cultured in a chemically defined medium. Proliferating colonies developed after 2-3 weeks. Three cell lines were established by cloning albumin-secreting colonies, as identified by an immunooverlay assay. Two of the cell lines,...

Topics

Animals
Biomarkers, Tumor
Cell Differentiation
Cell Line, Transformed
Genes, ras
Liver Neoplasms, Experimental
Mice
Mice, Nude
Phenotype
RNA, Messenger
Rats
Simian virus 40
Transfection

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