Article
Simian virus 40 large tumor antigen-immortalized normal human liver epithelial cells express hepatocyte characteristics and metabolize chemical carcinogens.
Proceedings of the National Academy of Sciences of the United States of America - 1 Jun 1993
Pfeifer A M, Cole K E, Smoot D T, Weston A, Groopman J D, Shields P G, Vignaud J M, Juillerat M, Lipsky M M, Trump B F
Abstract excerpt
Normal human liver tissue and cultured human hepatocytes are valuable models to study xenobiotic metabolism and toxicity, but they only have a limited in vitro life-span and are not readily available. This report describes the establishment of replicative cultures of human adult liver epithelial...
Topics
- Adult
- Antigens, Polyomavirus Transforming
- Biotransformation
- Blotting, Southern
- Carcinogens
- Cell Line, Transformed
- Cells, Cultured
- DNA
- Electron Transport Complex IV
- Epithelial Cells
- Epithelium
- Humans
- Karyotyping