Article
Development of an in vitro model of tumor progression using v-raf and v-raf/v-myc transformed rat liver epithelial cells: correlation of tumorigenicity with the downregulation of specific proteins.
Molecular carcinogenesis - 1 Jan 1990
Worland P J, Hampton L L, Thorgeirsson S S, Huggett A C
Abstract excerpt
A series of clonal cell lines were derived from rat liver epithelial cells after being infected with a defective retrovirus containing either v-raf (3611-MSV) or v-raf/v-myc(J2) together with a helper virus. These clones exhibited a different morphology from the regular cuboid shape of the contro...
Topics
- Animals
- Biomarkers, Tumor
- Blotting, Northern
- Blotting, Southern
- Cell Line, Transformed
- Cell Transformation, Neoplastic
- Cell Transformation, Viral
- Down-Regulation
- Electrophoresis, Gel, Two-Dimensional
- Gene Expression Regulation, Neoplastic
- Karyotyping
- Liver
- Liver Neoplasms, Experimental