Article
Poxvirus DNA topoisomerase knockout mutant exhibits decreased infectivity associated with reduced early transcription.
Proceedings of the National Academy of Sciences of the United States of America - 30 Sept 2003
Da Fonseca Flavio, Moss Bernard
Abstract excerpt
Vaccinia virus encodes a type I DNA topoisomerase that is highly conserved in all known poxviruses. Although the structure and catalytic activity of the enzyme were well studied, little was known about its biological function. The viral topoisomerase was thought to be essential, and roles in DNA replication, recombination, concatemer resolution, and transcription were suggested. Here, we demonstrated that the...
Topics
- DNA Topoisomerases, Type I
- DNA, Viral
- HeLa Cells
- Humans
- Microscopy, Electron
- Microscopy, Fluorescence
- Mutation
- Poxviridae
- Transcription, Genetic