Article
Predicted poxvirus FEN1-like nuclease required for homologous recombination, double-strand break repair and full-size genome formation.
Proceedings of the National Academy of Sciences of the United States of America - 20 Oct 2009
Senkevich Tatiana G, Koonin Eugene V, Moss Bernard
Abstract excerpt
Poxviruses encode many if not all of the proteins required for viral genome replication in the cytoplasm of the host cell. In this context, we investigated the function of the vaccinia virus G5 protein because it belongs to the FEN1-like family of nucleases and is conserved in all poxviruses. A vaccinia virus G5 deletion mutant was severely impaired, as the yield of infectious virus was reduced by approximately...
Topics
- Animals
- Base Sequence
- Catalytic Domain
- Cell Line
- Chlorocebus aethiops
- DNA Breaks, Double-Stranded
- DNA Repair
- DNA, Viral
- Flap Endonucleases
- Gene Deletion
- Genes, Viral
- Genetic Complementation Test
- Genome, Viral