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[Construction and expression of a new humanized salmon calcitonin variant].

Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae - 1 Aug 2001

Liu S J, Chen S S, Di X, Xiong A Q, Zhang Y H

Abstract excerpt

OBJECTIVE: To study the reduction of immunogenicity of salmon calcitonin (sCT). METHODS: A new (11-17) humanized salmon calcitonin variant gene (11-17) hsCT was designed and synthesized to construct recombinant expression vector and express in E.coli G1724 with tryptophan. The fusion protein of humanized salmon calcitonin and thioredoxin (Trx-hsCT) obtained from the bacterial by means of osmotic pressure; The...

Topics

Animals
Blotting, Western
Calcitonin
Escherichia coli
Genetic Variation
Genetic Vectors
Rats
Recombinant Fusion Proteins

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 12940075

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