Article
Phenotypic characterization of overexpression or deletion of the Escherichia coli crcA, cspE and crcB genes.
Microbiology (Reading, England) - 1 Aug 2003
Sand Olivier, Gingras Monica, Beck Nancy, Hall Christine, Trun Nancy
Abstract excerpt
The authors have previously shown that overexpression of the Escherichia coli K-12 crcA, cspE and crcB genes protects the chromosome from decondensation by camphor. In this study they examine the phenotypic consequences of deleting or overexpressing crcA, cspE and crcB. Overexpressing crcA, cspE and crcB increases supercoiling levels of plasmids in wild-type cells and in temperature-sensitive (Ts) gyrase mutants,...
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