Article
Characterisation of the ColE8 plasmid, a new member of the group E colicin plasmids.
Gene - 1 Jan 2000
Mark G, Lawrence P, James R
Abstract excerpt
We have determined the restriction map of the ColE8-J plasmid after cloning it into the pBR322 vector. By subcloning and transposon mutagenesis we have localized the colicin immunity gene, the colicin structural gene, and lys, the region that determines MC sensitivity. In contrast to the ColE3-CA38 plasmid, the genes coding for colicin E8 production and immunity cannot be cloned on a single EcoRI fragment....
Topics
- Bacteriocin Plasmids
- Base Sequence
- Cloning, Molecular
- Colicins
- DNA Restriction Enzymes
- DNA Transposable Elements
- Escherichia coli
- Genes
- Genes, Bacterial
- Mitomycin
- Mitomycins
- Phenotype