Overproduction of DnaE protein (alpha subunit of DNA polymerase III) restores viability in a conditionally inviable Escherichia coli strain deficient in DNA polymerase I.

doi:10.1128/jb.174.12.4166-4168.1992

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Article

Overproduction of DnaE protein (alpha subunit of DNA polymerase III) restores viability in a conditionally inviable Escherichia coli strain deficient in DNA polymerase I.

Journal of bacteriology - 1 Jun 1992

Witkin E M, Roegner-Maniscalco V

Abstract excerpt

A polA12 recA718 double mutant of Escherichia coli, in which DNA polymerase I is temperature sensitive, was unable to maintain normal DNA synthesis or to form colonies on rich media at 42 degrees C. Overproduction of DnaE protein, the polymerizing alpha subunit of DNA polymerase III, restored bac...

Topics

DNA Polymerase I
DNA Polymerase III
DNA Replication
DNA, Bacterial
Escherichia coli
Mutation
Plasmids
Rec A Recombinases
Temperature

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