Article
Gene mutation assay using a MutS protein-modified electrode.
Nucleic acids research. Supplement (2001) - 1 Jan 2002
Han Aishan, Shibata Taiki, Takarada Tohru, Maeda Mizuo
Abstract excerpt
A novel electrochemical biosensor for gene mutation detection was developed using a DNA mismatch recognizing protein MutS from E. coli. The MutS protein was immobilized onto an Au electrode by coordination of His-tag at its C-terminus to vacant sites of Ni(II)-nitrilotriacetato complex attached to the surface of electrode. When a target DNA duplex having a mismatch site was captured by the MutS protein on the...
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