Article
Deletion mutation analysis of the mutS gene in Escherichia coli.
The Journal of biological chemistry - 26 Feb 1999
Wu T H, Marinus M G
Abstract excerpt
The MutS protein is part of the dam-directed MutHLS mismatch repair pathway in Escherichia coli. We have constructed deletion derivatives in the mutS gene, which retain the P-loop coding region for ATP binding. The mutant proteins were assayed for ATP hydrolysis, heteroduplex DNA binding, heterodimer MutS formation, and the ability to interact with MutL. Dimerization was assayed by expressing His6-tagged...
Topics
- Adenosine Triphosphatases
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- DNA Repair
- DNA-Binding Proteins
- Escherichia coli
- Escherichia coli Proteins
- Molecular Sequence Data
- MutL Proteins
- MutS DNA Mismatch-Binding Protein
- Mutation
- Nucleic Acid Heteroduplexes
- Protein Binding
- Sequence Deletion