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Article

Immobilized MutS-Mediated Error Removal of Microchip-Synthesized DNA.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2017

Wan Wen, Wang Dongmei, Gao Xiaolian, Hong Jiong

Abstract excerpt

Applications of microchip-synthesized oligonucleotides for de novo gene synthesis are limited primarily by their high error rates. The mismatch binding protein MutS, which can specifically recognize and bind to mismatches, is one of the cheapest tools for error correction of synthetic DNA. Here, we describe a protocol for removing errors in microchip-synthesized oligonucleotides and for the assembly of DNA...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 27671944
DOI: 10.1007/978-1-4939-6343-0_17

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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