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MutS protein-based fiber optic particle plasmon resonance biosensor for detecting single nucleotide polymorphisms.

Analytical and bioanalytical chemistry - 1 May 2021

Ngo Loan Thi, Wang Wei-Kai, Tseng Yen-Ta, Chang Ting-Chou, Kuo Pao-Lin, Chau Lai-Kwan, Huang Tze-Ta

Abstract excerpt

A new biosensing method is presented to detect gene mutation by integrating the MutS protein from bacteria with a fiber optic particle plasmon resonance (FOPPR) sensing system. In this method, the MutS protein is conjugated with gold nanoparticles (AuNPs) deposited on an optical fiber core surface. The target double-stranded DNA containing an A and C mismatched base pair in a sample can be captured by the MutS...

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PMID: 33712917
DOI: 10.1007/s00216-021-03271-1

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