Article
Divergent signals and cytoskeletal assemblies regulate self-organizing polarity in neutrophils.
Cell - 25 Jul 2003
Xu Jingsong, Wang Fei, Van Keymeulen Alexandra, Herzmark Paul, Straight Aaron, Kelly Kathleen, Takuwa Yoh, Sugimoto Naotoshi, Mitchison Timothy, Bourne Henry R
Abstract excerpt
Like neutrophilic leukocytes, differentiated HL-60 cells respond to chemoattractant by adopting a polarized morphology, with F-actin in a protruding pseudopod at the leading edge and contractile actin-myosin complexes at the back and sides. Experiments with pharmacological inhibitors, toxins, and mutant proteins show that this polarity depends on divergent, opposing "frontness" and "backness" signals generated by...
Topics
- Cell Polarity
- Chemotaxis, Leukocyte
- Cytoskeleton
- HL-60 Cells
- Humans
- Models, Biological
- Mutation
- Myosin Type II
- N-Formylmethionine Leucyl-Phenylalanine
- Neutrophils
- Pertussis Toxin
- Phosphoinositide-3 Kinase Inhibitors
- Protein Serine-Threonine Kinases