Single-base mutations at position 2661 of Escherichia coli 23S rRNA increase efficiency of translational proofreading.

doi:10.1128/jb.174.24.7896-7901.1992

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Article

Single-base mutations at position 2661 of Escherichia coli 23S rRNA increase efficiency of translational proofreading.

Journal of bacteriology - 1 Dec 1992

Melançon P, Tapprich W E, Brakier-Gingras L

Abstract excerpt

Two single-base substitutions were constructed in the 2660 loop of Escherichia coli 23S rRNA (G2661-->C or U) and were introduced into the rrnB operon cloned in plasmid pKK3535. Ribosomes were isolated from bacteria transformed with the mutated plasmids and assayed in vitro in a poly(U)-directed...

Topics

Base Sequence
Codon
Escherichia coli
Frameshift Mutation
Gentamicins
Molecular Sequence Data
Mutation
Neomycin
Protein Biosynthesis
RNA, Bacterial
RNA, Ribosomal, 23S
Ribosomes
Streptomycin
Suppression, Genetic

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