Article
Analysis of mutations at residues A2451 and G2447 of 23S rRNA in the peptidyltransferase active site of the 50S ribosomal subunit.
Proceedings of the National Academy of Sciences of the United States of America - 31 Jul 2001
Thompson J, Kim D F, O'Connor M, Lieberman K R, Bayfield M A, Gregory S T, Green R, Noller H F, Dahlberg A E
Abstract excerpt
On the basis of the recent atomic-resolution x-ray structure of the 50S ribosomal subunit, residues A2451 and G2447 of 23S rRNA were proposed to participate directly in ribosome-catalyzed peptide bond formation. We have examined the peptidyltransferase and protein synthesis activities of ribosomes carrying mutations at these nucleotides. In Escherichia coli, pure mutant ribosome populations carrying either the...
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