Mutations in 23 S ribosomal RNA perturb transfer RNA selection and can lead to streptomycin dependence.

Abstract excerpt

Escherichia coli ribosomes with a G to C transversion at position 2661 in 23 S ribosomal RNA are more accurate in tRNA selection than wild-type ribosomes. This enhanced accuracy is due to improved initial selection of ternary complexes rather than proofreading of aminoacyl tRNAs. The 2661C mutation reduces the binding rate of cognate ternary complexes to the A-site. This binding rate deficiency becomes dramatic...

Topics

• Escherichia coli
• Guanosine Triphosphate
• Mutation
• Peptide Elongation Factor G
• Peptide Elongation Factor Tu
• Peptide Elongation Factors
• Peptidyl Transferases
• Protein Biosynthesis
• RNA, Bacterial
• RNA, Ribosomal, 23S
• RNA, Transfer