Article
A ribosomal ambiguity mutation in the 530 loop of E. coli 16S rRNA.
Nucleic acids research - 25 Aug 1992
O'Connor M, Göringer H U, Dahlberg A E
Abstract excerpt
A series of base substitution and deletion mutations were constructed in the highly conserved 530 stem and loop region of E. coli 16S rRNA involved in binding of tRNA to the ribosomal A site. Base substitution and deletion of G517 produced significant effects on cell growth rate and translational fidelity, permitting readthrough of UGA, UAG and UAA stop codons as well as stimulating +1 and -1 frameshifting in...
Topics
- Base Sequence
- DNA Mutational Analysis
- Escherichia coli
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Plasmids
- Protein Biosynthesis
- RNA, Bacterial
- RNA, Ribosomal, 16S
- Recombinant Proteins