Article
Electrophoretic detection of single-nucleotide polymorphisms.
BioTechniques - 1 Apr 2000
See D, Kanazin V, Talbert H, Blake T
Abstract excerpt
Single-nucleotide polymorphisms (SNPs) represent the most prevalent class of genetic markers available for linkage disequilibrium or cladistic analyses. PCR primers may be labeled with fluorescent dyes and used to rapidly and accurately differentiate among alleles that are defined by a single-nucleotide differences. Here, we describe the primer-mediated detection of SNPs based on primer mismatch during...
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