Article

Deep profiling of protease substrate specificity enabled by dual random and scanned human proteome substrate phage libraries

2020-05-10

Abstract excerpt

Proteolysis is a major post-translational regulator of biology both inside and outside of cells. Broad identification of optimal cleavage sites and natural substrates of proteases is critical for drug discovery and to understand protease biology. Here we present a method that employs two genetically encoded substrate phage display libraries coupled with next generation sequencing (SPD-NGS) that allows up to 10,000...

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Identifiers and source

Literature Corpus work: 1434e13e-4036-5f9e-8520-ac5ca40aac55
DOI: 10.1101/2020.05.09.086264

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