Phage display of proteases and macromolecular inhibitors.

doi:10.1016/s0076-6879(96)67005-0

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Article

Phage display of proteases and macromolecular inhibitors.

Methods in enzymology - 1 Jan 1996

Wang C I, Yang Q, Craik C S

Abstract excerpt

We describe methods for displaying the protease trypsin and the macromolecular protease inhibitor ecotin on the surface of filamentous phage. Our strategy for selecting variant ecotins against target proteases is also described. We believe that the two proteins that have been displayed serve as i...

Topics

Bacterial Proteins
Bacteriophage M13
Base Sequence
Escherichia coli Proteins
Genetic Variation
Genetic Vectors
Molecular Sequence Data
Periplasmic Proteins
Protein Binding
Protein Engineering
Selection, Genetic
Structure-Activity Relationship
Trypsin
Trypsin Inhibitors

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