Article
[Basic studies on mutation analysis of K-ras codon 12 by use of three-dimensional microarray system].
Rinsho byori. The Japanese journal of clinical pathology - 1 Apr 2003
Maekawa Masato, Taniguchi Terumi, Tatebayashi Chika, Horii Toshinobu, Takeshita Akihiro, Sugimura Haruhiko, Sugano Kokichi, Yonekawa Hiroyuki, Nagaoka Tomonori, Kanno Takashi
Abstract excerpt
A next-generation DNA microarray system, FD10 has been developed. It is based around the PamChip, a custom-made microarray, which consists of a solid three-dimensional structure that facilitated the incorporation of probe molecules. We applied this microarray system on a detection of K-ras mutation at codon 12 in some cancer cell lines. The PCR products amplified by use of FITC labeled primers were applied onto...
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