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Article

Polyacrylamide gel film immobilized molecular beacon array for single nucleotide mismatch detection.

Journal of nanoscience and nanotechnology - 1 Apr 2005

Wang Yijin, Wang Hong, Gao Lu, Liu Heping, Lu Zuhong, He Nongyue

Abstract excerpt

We reported polyacrylamide gel immobilized molecular beacon array for single nucleotide mismatch detection in this paper. Molecular beacons are oligonucleotide probes fluorescing upon hybridization to their complementary DNA/RNA targets with excellent sensitivity and high selectivity. The specially designed molecular beacon for immobilization contains a 15 base loop sequence with a 5 base pair stem, a polyT (20...

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PMID: 16004134
DOI: 10.1166/jnn.2005.086

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