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[Establishment of microarray for detecting mutation in HBV pre-core/core and basic core promoter regions].

Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology - 1 Oct 2010

Fang Li-juan, Lai Le-xiang, Le Yi-quan, Ren Ling-jun

Abstract excerpt

OBJECTIVE: To develop a sensitive and specific microarray for detecting mutations of HBV pre-core/core and basic core promoter regions in the clinic. METHODS: Site-specific oligonucleotide probes were designed and immobilized to microarray slides and hybridized to HBV gene fragments amplified with specific biotin-labeled primer using asymmetrical PCR. The specificity and sensitivity of the method were estimated....

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 21280334

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