Article
Impact of DNA ligase IV on the fidelity of end joining in human cells.
Nucleic acids research - 15 Apr 2003
Smith Julianne, Riballo Enriqueta, Kysela Boris, Baldeyron Celine, Manolis Kostas, Masson Christel, Lieber Michael R, Papadopoulo Dora, Jeggo Penny
Abstract excerpt
A DNA ligase IV (LIG4)-null human pre-B cell line and human cell lines with hypomorphic mutations in LIG4 are significantly impaired in the frequency and fidelity of end joining using an in vivo plasmid assay. Analysis of the null line demonstrates the existence of an error-prone DNA ligase IV-independent rejoining mechanism in mammalian cells. Analysis of lines with hypomorphic mutations demonstrates that...
Topics
- Base Pairing
- Cell Line
- Cells, Cultured
- DNA Ligase ATP
- DNA Ligases
- DNA Repair
- DNA-Binding Proteins
- Humans
- Mutation
- Plasmids