Article
DNA ligase IV and artemis act cooperatively to suppress homologous recombination in human cells: implications for DNA double-strand break repair.
PloS one - 1 Jan 2013
Kurosawa Aya, Saito Shinta, So Sairei, Hashimoto Mitsumasa, Iwabuchi Kuniyoshi, Watabe Haruka, Adachi Noritaka
Abstract excerpt
Nonhomologous end-joining (NHEJ) and homologous recombination (HR) are two major pathways for repairing DNA double-strand breaks (DSBs); however, their respective roles in human somatic cells remain to be elucidated. Here we show using a series of human gene-knockout cell lines that NHEJ repairs nearly all of the topoisomerase II- and low-dose radiation-induced DNA damage, while it negatively affects survival of...
Topics
- Cell Line
- DNA Breaks, Double-Stranded
- DNA End-Joining Repair
- DNA Ligase ATP
- DNA Ligases
- DNA Replication
- DNA Topoisomerases, Type II
- DNA-Binding Proteins
- Endonucleases
- Gene Knockout Techniques
- Gene Targeting
- Homologous Recombination
- Humans