Article
Mutation of an N-terminal acidic-rich region of p115-RhoGEF dissociates alpha13 binding and alpha13-promoted plasma membrane recruitment.
FEBS letters - 10 Apr 2003
Bhattacharyya Raja, Wedegaertner Philip B
Abstract excerpt
The Ras homology (Rho) guanine nucleotide exchange factor p115-RhoGEF couples the alpha(13) heterotrimeric guanine nucleotide binding protein (G protein) subunit to Rho GTPase. Alpha(13) binds to a regulator of G protein signaling (RGS) domain in p115-RhoGEF, but the mechanism of alpha(13) activation of p115-RhoGEF is poorly understood. In this report, we demonstrate in cell-based assays that the acidic-rich...
Topics
- Aluminum Compounds
- Amino Acid Sequence
- Blotting, Western
- Cell Line
- Cell Membrane
- Fluorides
- GTP-Binding Proteins
- Guanine Nucleotide Exchange Factors
- Humans
- Molecular Sequence Data
- Mutation
- Precipitin Tests
- Rho Guanine Nucleotide Exchange Factors
- Sequence Deletion