Article
An amplification and ligation-based method to scan for unknown mutations in DNA.
Human mutation - 1 Aug 2002
Zhang Yuzhi, Kaur Manjit, Price Brendan D, Tetradis Sotirios, Makrigiorgos G Mike
Abstract excerpt
A new approach is presented for the sensitive and selective scanning for unknown DNA mutations, based on ligation-mediated PCR and the use of the glycosylases TDG and MutY. These two highly selective enzymes together can detect about 70% of commonly observed polymorphisms and mutations in human tumors. DNA is cross-hybridized to form mismatches at the positions of point mutations, de-phosphorylated to eliminate...
Topics
- Adenine
- Antigens, Nuclear
- Ataxia Telangiectasia Mutated Proteins
- Bacteriophage T4
- Base Pair Mismatch
- Cell Cycle Proteins
- DNA Helicases
- DNA Ligases
- DNA Mutational Analysis
- DNA-Binding Proteins
- Genes, p53
- Guanine
- Humans
- Ku Autoantigen