Article
[Role of "anti-restriction" motif in functional activity of anti-restriction protein ArdA pKM101 (incN)].
Genetika - 1 Feb 2003
Rastorguev S M, Zavil'gel'skiĭ G B
Abstract excerpt
A number of mutant forms of the antirestriction protein ArdA encoded by the ardA gene located in a transmissive IncN plasmid pKM101 have been constructed. Proteins belonging to the Ard family are specific inhibitors of type I restriction--modification enzymes. Single mutational substitutions of negatively charged amino acid residues located in the "antirestriction motif" with hydrophobic alanine, E134A, E137A,...
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