Article
[Alleviation of type I restriction in the presence of plasmids of group inc I. General characteristics and molecular cloning of the ard gene].
Molekuliarnaia biologiia - 1 Jan 2000
Kotova V Iu, Zavil'gel'skiĭ G B, Belogurov A A
Abstract excerpt
The capability of a number of plasmids of incN and incI groups to alleviate an action of type I EcoK, EcoB, EcoD, and EcoA restriction endonucleases on the unmodified DNA was revealed. The efficiency of EcoK action on lambda 0 DNA is alleviated about 10 divided by 100 fold in E. coli K12 AB 1157 bacteria containing the plasmid of incN group (pKM101, N3, pJA4733) or incI group (R144, R648; R621a; ColIb-P9). We...
Topics
- Bacteriophage lambda
- Cloning, Molecular
- DNA Restriction Enzymes
- Escherichia coli
- Genes, Bacterial
- Phenotype
- Plasmids
- Transformation, Bacterial
