Article
[Weakening of bacteriophage lambda EcoK DNA restriction in the presence of plasmid pKM101 ard+. I. General characteristics and genetic localization].
Molekuliarnaia biologiia - 1 Jan 2000
Zavil'gel'skiĭ G B, Mershavka V Iu, Iusifov T N, Belogurov A A
Abstract excerpt
The host-controlled K-restriction of unmodified phage lambda is ten to hundred-fold alleviated in the E. coli K12 strain, carring plasmid pKM101 of N-incompatibility group. By restriction mapping Tn5 insertion in pKM101, which reduced pKM101-mediated alleviation of K-restriction, was shown to by...
Topics
- Bacteriophage lambda
- Chromosome Mapping
- DNA Restriction Enzymes
- DNA, Bacterial
- DNA, Viral
- Deoxyribonucleases, Type I Site-Specific
- Escherichia coli
- Genes, Bacterial
- Genetic Markers
- Mutation
- Phenotype
- Plasmids