[Reduced restriction of EcoK in the presence of the plasmid pKM ard+. II. Cloning of the gene ard].

Article

[Reduced restriction of EcoK in the presence of the plasmid pKM ard+. II. Cloning of the gene ard].

Molekuliarnaia biologiia - 1 Jan 2000

Iusifov T N, Zavil'gel'skiĭ G B, Del'ver E P, Belogurov A A

Abstract excerpt

Plasmid pKM101 affects the I type restriction EcoK in E. coli. The gene ard responsible for alleviation of EcoK restriction was shown to be located within the BglIIB fragment of pKM101. Here we have cloned ard gene into high copy vectors pBR322 and pUC12. Plasmid pD12 was constructed by introduci...

Topics

Chromosome Mapping
Cloning, Molecular
DNA Restriction Enzymes
Deoxyribonucleases, Type I Site-Specific
Escherichia coli
Genes, Bacterial
Mutation
Plasmids

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