Article
Isolation of a non-classical mutant of the DNA recognition subunit of the type I restriction endonuclease R.EcoR124I.
Biological chemistry - 1 Jan 2000
Weiserova M, Firman K
Abstract excerpt
We have used deletion mutagenesis and PCR-based misincorporation mutagenesis to produce a collection of mutations in the central conserved region of the DNA binding subunit of the type IC restriction endonuclease EcoR124I. It has been proposed that this domain is involved in protein-protein inter...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Binding Sites
- DNA
- DNA Restriction-Modification Enzymes
- Deoxyribonucleases, Type I Site-Specific
- Molecular Sequence Data
- Mutagenesis
- Mutation