Article
Conditional gene expression by controlling translation with tetracycline-binding aptamers.
Nucleic acids research - 1 Apr 2003
Suess Beatrix, Hanson Shane, Berens Christian, Fink Barbara, Schroeder Renée, Hillen Wolfgang
Abstract excerpt
We present a conditional gene expression system in Saccharomyces cerevisiae which exploits direct RNA-metabolite interactions as a mechanism of genetic control. We inserted preselected tetracycline (tc) binding aptamers into the 5'-UTR of a GFP encoding mRNA. While aptamer insertion generally reduces GFP expression, one group of aptamers displayed an additional, up to 6-fold, decrease in fluorescence upon tc...
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