Article
Construction of synthetic T7 RNA polymerase expression systems.
Methods (San Diego, Calif.) - 1 Jul 2018
Kar Shaunak, Ellington Andrew D
Abstract excerpt
T7 RNA polymerase (T7 RNAP) is one of the preferred workhorses for recombinant gene expression, owing in part to its high transcriptional activity and the fact that it has a small (17 base-pair), easily manipulated promoter. Furthermore, the fact that T7 RNAP is largely orthogonal to most hosts enables its use in a wide variety of contexts. However, the high activity of the enzyme also often leads to an increased...
Topics
- Cloning, Molecular
- DNA-Directed RNA Polymerases
- Electroporation
- Escherichia coli
- Green Fluorescent Proteins
- Models, Biological
- Mutation
- Plasmids
- Promoter Regions, Genetic
- Recombinant Proteins
- Synthetic Biology