Article
Continuous control of the flow in biochemical pathways through 5' untranslated region sequence modifications in mRNA expressed from the broad-host-range promoter Pm.
Applied and environmental microbiology - 1 Apr 2011
Lale Rahmi, Berg Laila, Stüttgen Friederike, Netzer Roman, Stafsnes Marit, Brautaset Trygve, Vee Aune Trond Erik, Valla Svein
Abstract excerpt
The inducible Pm promoter integrated into broad-host-range plasmid RK2 replicons can be fine-tuned continuously between the uninduced and maximally induced levels by varying the inducer concentrations. To lower the uninduced background level while still maintaining the inducibility for applications in, for example, metabolic engineering and synthetic (systems) biology, we report here the use of mutations in the...
Topics
- 5' Untranslated Regions
- Down-Regulation
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Genes, Reporter
- Luciferases
- Micrococcus luteus
- Mutagenesis, Site-Directed
- Mutation