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MPD: multiplex primer design for next-generation targeted sequencing.

BMC bioinformatics - 5 Jan 2017

Wingo Thomas S, Kotlar Alex, Cutler David J

Abstract excerpt

BACKGROUND: Targeted resequencing offers a cost-effective alternative to whole-genome and whole-exome sequencing when investigating regions known to be associated with a trait or disease. There are a number of approaches to targeted resequencing, including microfluidic PCR amplification, which may be enhanced by multiplex PCR. Currently, there is no open-source software that can design next-generation multiplex...

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PMID: 28056760
DOI: 10.1186/s12859-016-1453-3
PMCID: PMC5217220

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