Article
Genotyping single-nucleotide polymorphisms by minisequencing using tag arrays.
Methods in molecular medicine - 1 Jan 2005
Lovmar Lovisa, Syvänen Ann-Christine
Abstract excerpt
The need for large-scale and high-throughput methods for SNP genotyping has rapidly increased during the last decade. Our system, presented here, combines the highly specific genotyping principle of minisequencing with the advantages of a microarray format that allows highly multiplexed and parallel analysis. Cyclic minisequencing reactions with fluorescently labeled dideoxynucleotides (ddNTPs) are performed in...
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