Article
Primer design for multiplexed genotyping.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2007
Kaderali Lars
Abstract excerpt
Single-nucleotide polymorphism (SNP) genotyping can be carried out by annealing an oligonucleotide primer directly adjacent to the polymorphism and carrying out a single base extension using a polymerase reaction with labeled dideoxynucleotide triphosphates. This can be multiplexed by attaching a unique tag at the 5'-end of each oligonucleotide primer and binding the corresponding antitag to a DNA microarray or...
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