Article
RecFOR function is required for DNA repair and recombination in a RecA loading-deficient recB mutant of Escherichia coli.
Genetics - 1 Feb 2003
Ivancić-Baće Ivana, Peharec Petra, Moslavac Suncana, Skrobot Nikolina, Salaj-Smic Erika, Brcić-Kostić Krunoslav
Abstract excerpt
The RecA loading activity of the RecBCD enzyme, together with its helicase and 5' --> 3' exonuclease activities, is essential for recombination in Escherichia coli. One particular mutant in the nuclease catalytic center of RecB, i.e., recB1080, produces an enzyme that does not have nuclease activity and is unable to load RecA protein onto single-stranded DNA. There are, however, previously published contradictory...
Topics
- DNA Repair
- DNA, Single-Stranded
- Escherichia coli
- Escherichia coli Proteins
- Exodeoxyribonuclease V
- Exodeoxyribonucleases
- Gamma Rays
- Mutation
- Rec A Recombinases
- Recombination, Genetic
- Ultraviolet Rays