Article
Balancer-Cre transgenic mouse germ cells direct the incomplete resolution of a tri-loxP-targeted Cyp1a1 allele, producing a conditional knockout allele.
Biochemical and biophysical research communications - 12 Dec 2003
Uno Shigeyuki, Wang Bin, Shertzer Howard G, Nebert Daniel W, Dalton Timothy P
Abstract excerpt
To generate conditional alleles, genes are commonly engineered to contain recognition sites for bacteriophage recombinases, such as Cre recombinase. When such motifs (lox sites) flank essential gene sequences, and provided that Cre recombinase is expressed, Cre recombinase will excise the flanked sequence-creating a conditional knockout allele. Targeted conditional alleles contain a minimum of three lox sites. It...
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